Figure 3.

IL-8 production in human primary FLSs stimulated with PLA1A. Human primary FLSs from normal donors were stimulated with 0.2 µg/mL or 0.5 µg/mL recombinant PLA1A for 24 h. The amounts of IL-8 released in the supernatants were monitored using ELISA. IL-8 produced by FLSs cultured in serum-free DMEM was 4.03 ± 1.53 pg/mL, and for each experiment, basal IL-8 production was set at 100% for data normalization. The data shown are the means ± SE from three experiments. For statistical comparative analyses, cytokine amount in cells stimulated with PLA1A were compared to that in non-stimulated cells. ** p < 0.01.