Figure 6.

IL-8 production in human primary FLSs stimulated with exogenous PLA1A and ATX. Human primary FLSs from normal donors were stimulated with 0.2 µg/mL recombinant PLA1A in combination with 5 nM recombinant ATX for 24 h, in the absence (A) or presence (B) of 1% fatty acid-free BSA added in the culture medium. Amounts of IL-8 released in the supernatants were monitored using ELISA. IL-8 produced by FLSs cultured in serum-free DMEM supplemented with or without 1% fatty acid-free BSA was 7.08 ± 2.45 and 8.05 ± 2.04 pg/mL, respectively. For each experiment, basal IL-8 production was set at 100% for data normalization. The data are the means ± SE from five (A) and three (B) experiments, respectively. For statistical comparative analyses, cytokine amounts in cells stimulated with PLA1A and/or ATX were compared to that in non-stimulated cells. ** p < 0.01.