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. 2021 Nov 30;22(23):12982. doi: 10.3390/ijms222312982

Figure 1.

Figure 1

(A) Changes in the absorption spectrum due to oxidation of a boronate probe (FlBA) in an aerated aqueous solution of Angeli’s salt in the absence of HNO scavengers. (B) An example of the effect of an HNO scavenger 2,4-piperidinedione on the initial rate of fluorescein formation from the FlBA boronate probe. (C) Example plot of (v0/vi) − 1 versus the [S]/[O2] ratio. The reaction mixture contained: 25 µM FlBA, 20 µM Angeli’s salt, 2,4-piperidinedione (0–250 µM), phosphate buffer (pH 7.4, 50 mM), dtpa (100 µM). Each solution contained 5% (vol.) CH3CN. The concentration of molecular oxygen was assumed to be 225 µM. Measurements were carried out at 25 °C.