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. 2021 Nov 25;22(23):12735. doi: 10.3390/ijms222312735

Figure 9.

Figure 9

Effect of anti-androgen flutamide treatment on wild-type (WT) and genetically modified mice. (A,B): testicular histology and macroscopic views of the testes and urogenital blocks of WT and Fshr-CAM/LuRKO mice (A): the treatment arrested spermatogenesis at round spermatid stage in WT mice and reduced their testis and seminal vesicle sizes. (B) identical treatment of Fshr-CAM/LuRKO mice had no apparent effect on their spermatogenesis and testis size but reduced seminal vesicle size (arrows in (B)). (C,D): expression of selected target genes in untreated (A) and flutamide-treated (B) mice. (A): expression of androgen-regulated (Drd5, Rhox5, Eppin, and Tjp1), postmeiotic germ cell–specific (Aqp8), and germ cell–regulated (Gata1) genes in WT, Fshr-CAM, Fshr-CAM/LuRKO and LuRKO testes. (B): effect of flutamide treatment on expression of the same androgen-regulated genes in WT and Fshr-CAM/LuRKO mice. Data represent mean ± SEM. n = 3 samples/group. Bars with different symbols (*, **, #) differ significantly from each other (p < 0.05; ANOVA/Newman-Keuls). The remarkable finding is that while flutamide treatment suppressed the expression of strictly androgen-dependent genes in WT mice, the same effect was not observed in the testis of Fshr-CAM/LuRKO mice. Scale bars: 50 μm. From [133] with permission.