FIG. 3.

(A–C) Comparative analysis of extended type IV loci in Fervidacidithiobacillus caldus strains. The extended type IV-A gene neighborhoods from integrative and conjugative elements from closely related F. caldus strains are mapped to the cas6 nucleotide sequence-based tree, which was constructed using FastTree. The designations are the same as in the Figure 1. The color key and names or short descriptions of the respective genes are given underneath the schematic. A few rare genes that were inserted in the closest vicinity of type IV genes are indicated in orange above the respective blank arrows. Gray dashed lines indicate the regions that are aligned in Supplementary Figure S7.