TABLE 1.

Primers and conditions for PCR of transmissibility marker genes of B. cepacia

Target	Primer (reference)	Sequence	PCR conditions	Amplicon size (bp)
cblA	CBL1 (10)	5′ CCAAAGGACTAACCCA 3′	96°C for 5 min, 1 cycle	664
	CBL2	5′ AGCCGATGTCCATCACA 3′	96°C for 15 s, 58°C for 30 s, 72°C for 90 s, 25 cycles; 70°C for 5 min, 1 cycle
IS hybrid	402A (11)	5′ CAACCGAGACTGAGGAGATG 3′	96°C for 5 min, 1 cycle	592
	1356B	5′ TCCGGCGACACCTCGATGCC 3′	96°C for 15 s, 63°C for 30 s, 72°C for 90 s, 25 cycles; 70°C for 5 min, 1 cycle
Epidemic marker	BCESM1 (13)	5′ CCACGGACGTGACTAACA 3′	96°C for 5 min, 1 cycle	1,400
	BCESM2	5′ CGTCCATCCGAACACGAT 3′	96°C for 15 s, 63°C for 30 s, 72°C for 90 s, 25 cycles; 70°C for 5 min, 1 cycle
RAPD	P272 (12)	5′ AGCGGGCCAA 3′	95°C for 5 min, 1 cycle; 95°C for 1 min, 36°C for 5 min, 72°C for 2 min, 45 cycles