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Meiotic CO designation and maturation appears normal in topII plants. (A) HEI10 (green) and ASY1 (red) staining of wt, topII-1 and topII-RNAi zygotene cells and HEI10 foci distribution per cell. (B) MLH1 (green) and ZYP1 (red) staining of wt, topII-1 and topII-RNAi pachytene cells and MLH1 foci distribution per cell. Bars: mean ± standard deviation. Mann–Whitney U-test, p-value: ** <0.01.
