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Impact of TCRβ transgene on T-cell development in SCID mice. Thymocytes from two 4-day-old TCRβ-SCID mice and a nontransgenic littermate were evaluated for CD4 and CD8 expression by flow cytometry. One TCRβ-SCID mouse treated with 100 cGy of γ-irradiation 24 h prior to this analysis is also shown for comparison. The cellularity of each thymus is indicated. The absolute number of CD25+ DN cells in each sample did not vary by more than twofold.
