TABLE 5.
Effect of TCRβ transgene on proliferation of SCID thymocytesa
| Treatment group | Age (weeks) | Tg | n | % (± SD) S/G2/M |
|---|---|---|---|---|
| Not irradiated | 2 | − | 3 | 24.7 ± 5.6 |
| + | 7 | 32.6 ± 1.0 | ||
| Irradiated | 6 | − | 3 | 43.7 ± 5.4 |
| + | 4 | 46.3 ± 4.5 | ||
| Irradiated | 10 | − | 3 | 22.9 ± 4.0 |
| + | 3 | 29.4 ± 2.2 |
a
Effect of the TCRβ transgene (Tg) on the proliferative status of SCID thymocytes. Three litters from a TCRβ-SCID × SCID mating were irradiated with 100 cGy within 48 h after birth or were left untreated. Mice were sacrificed at 2 to 10 weeks of age, and the percentage of cells in the S, G2, and M phases of the cell cycle was quantitated by flow cytometric analysis of propidium iodide-stained thymocytes.