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. 2021 Dec 3;22(23):13106. doi: 10.3390/ijms222313106

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Timeline of the study interventions (e.g., pump implantation for i.t. growth factor (GF) delivery at day 10 or manganese-enhanced magnetic resonance imaging (MEMRI) at day 56), also indicating the group design and the group sizes at the beginning and at the end of the experiment. (B) The contusion-compression SCI is performed at day 1 with a modified aneurysm clip (C), leaving a visible injury on the exposed cervical spinal cord at the C6 level (D). (E) Modified after (47)—NPCs are transplanted into the spinal cord at four distinct sites via stereotactic injection (F) at day 10.