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(A,B) FTIR spectrum of a flat uncoated alginate hydrogel (ALG) and a three-layer layer-by-layer coating collagen-hyaluronic acid-collagen (COL-HA-COL); (C) Collagen content in the layer-by-layer coating by adding layers of collagen and hyaluronic acid successively, as determined by Micro-BCA. ALG: alginate hydrogel; COL: collagen; HA: hyaluronic acid.
