TABLE 1.
PCR amplification of different mycobacterial and nonmycobacterial species using the pan-Mycobacterium primers
| Speciesa | No. of isolates | Pan-Mycobacterium amplification resultc |
|---|---|---|
| Mycobacterium | ||
| M. asiaticum ATCC 25276 | 1 | + |
| M. avium ATCC 15769 | 1 | + |
| M. fortuitum ATCC 6841 | 1 | + |
| M. gordonae ATCC 14470 | 1 | + |
| M. haemophilum ATCC 43160 | 1 | + |
| M. intracellulare ATCC 13950 | 1 | + |
| M. marinum ATCC 11565 | 1 | + |
| M. phlei ATCC 11758 | 1 | + |
| M. scrofulaceum ATCC 19981 | 1 | + |
| M. smegmatis ATCC 19420 | 1 | + |
| M. tuberculosis ATCC 27294 | 1 | + |
| M. aviumb | 20 | + |
| M. bovisb | 1 | + |
| M. chelonaeb | 1 | + |
| M. kansasiib | 1 | + |
| M. tuberculosisb | 20 | + |
| M. xenopib | 1 | + |
| Other bacteria and fungusa | ||
| Acinetobacter calcoaceticus ATCC 23055 | 1 | − |
| Citrobacter freundii ATCC 8090 | 1 | − |
| Enterobacter cloacae ATCC 13047 | 1 | − |
| Neisseria meningitidis ATCC 13102 | 1 | − |
| Candida albicansb | 1 | − |
| Escherichia colib | 1 | − |
| Haemophilus influenzaeb | 1 | − |
| Klebsiella pneumoniaeb | 1 | − |
| Proteus mirabilisb | 1 | − |
| Pseudomonas aeruginosab | 1 | − |
| Staphylococcus aureusb | 1 | − |
| Staphylococcus epidermidisb | 1 | − |
| Streptococcus pneumoniaeb | 1 | − |
a
Mycobacterial and bacterial species and a fungus used to determine the coverage of primers M-OU-1 and M-OL-2.
b
Clinical isolates from the University of Milan, Milan, Italy.
c
Amplification results were determinated by gel electrophoresis. Symbols: +, presence of amplification products; −, absence of amplification products.