Figure 5.

Detection of CpG methylation in a major depressive disorder (MDD) marker. Cg26910488_comp is unmethylated, while cg26910488_comp M1 (including one 5 mC) and cg26910488_comp M5 (including five 5 mCs) are methylated. (a) Circular dichroism (CD) spectra of 2 µM cg26910488_comp, cg26910488_comp M1, and cg26910488_comp M5 in 10 mM Tris-HCl, 100 mM KCl, pH 7.4. (b) The biding assay of L1Cy5-7OTD to cg26910488_comp and cg26910488_comp M5. Binding abilities of L1Cy5-7OTD to DNAs were determined by chemiluminescence intensities. In the binding assay, as a negative control, a well without immobilized DNA (None) was utilized. Bar graphs represent mean values of the chemiluminescence intensities and the error bars represent the standard deviation (n = 3). Statistical analyses were performed using t-test. The significance of differences of observed values are indicated as ** p < 0.001.