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. 2001 Jan;21(2):414–424. doi: 10.1128/MCB.21.2.414-424.2001

TABLE 3.

Activation of α-fibrinogen and G6Pase promoters in HeLa cells with HNF-1α and IL-6/STAT3/c-Fos cotransfectiona

Promoter Cotransfection
Luciferase activity
HNF-1α DN-STAT3 STAT3/IL-6 c-Fos Fold (avg) SD
G6Pase 1.0 0.3
+ 1.0 0.3
+ 1.6 0.1
+ 1.8 0.0
+ + 3.1 0.3
+ 4.7 0.8
+ + 2.1 0.3
+ + 8.1 2.1
+ + + 2.4 0.4
+ + 10.2 0.4
+ + + 11.3 1.3
αFibrinogen 1.0 0.2
+ 1.0 0.0
+ 1.5 0.0
+ 1.0 0.4
+ + 1.5 0.4
+ 14.1 0.2
+ + 3.1 0.6
+ + 19.4 0.1
+ + + 2.4 0.4
+ + 30.6 1.1
+ + + 69.5 5.5
AP-1 1.0 0.1
+ 0.2 0.0
+ 1.1 0.0
+ + 0.4 0.0
PRL-1 pP1-Sma 1.0 0.1
+ 1.4 0.2
+ 1.1 0.2
+ + 0.7 0.1
HRS/SRp40 pGL0.1 1.0 0.2
+ 0.5 0.1
+ 2.0 0.4
+ + 0.3 0.0
SMAD7 1.0 0.1
+ 1.1 0.2
+ 1.6 0.4
+ + 1.5 0.4
a

HeLa cells were transfected with the indicated reporter constructs (50 ng) in 24-well plates. For cotransfection experiments, 50 ng of pCMV-STAT3, 50 ng of pCMV-c-fos, 100 ng of DN-STAT3, or 50 ng of pcDNA-HNF-1α (aa 1 to 628, full length) was (+) or was not (−) used. The transfected cells were treated with IL-6 (100 ng/ml) for 4 h. Luciferase activity was expressed as fold induction relative to the basal activity of the reporter construct in the absence of IL-6 treatment and in the absence of any indicated expression plasmids. Nine independent determinants were made for each construct by performing triplicates in three separate experiments.