TABLE 3.
Activation of α-fibrinogen and G6Pase promoters in HeLa cells with HNF-1α and IL-6/STAT3/c-Fos cotransfectiona
Promoter | Cotransfection
|
Luciferase activity
|
||||
---|---|---|---|---|---|---|
HNF-1α | DN-STAT3 | STAT3/IL-6 | c-Fos | Fold (avg) | SD | |
G6Pase | − | − | − | − | 1.0 | 0.3 |
− | + | − | − | 1.0 | 0.3 | |
− | − | − | + | 1.6 | 0.1 | |
− | − | + | − | 1.8 | 0.0 | |
− | − | + | + | 3.1 | 0.3 | |
+ | − | − | − | 4.7 | 0.8 | |
+ | + | − | − | 2.1 | 0.3 | |
+ | − | − | + | 8.1 | 2.1 | |
+ | + | − | + | 2.4 | 0.4 | |
+ | − | + | − | 10.2 | 0.4 | |
+ | − | + | + | 11.3 | 1.3 | |
αFibrinogen | − | − | − | − | 1.0 | 0.2 |
− | + | − | − | 1.0 | 0.0 | |
− | − | − | + | 1.5 | 0.0 | |
− | − | + | − | 1.0 | 0.4 | |
− | − | + | + | 1.5 | 0.4 | |
+ | − | − | − | 14.1 | 0.2 | |
+ | + | − | − | 3.1 | 0.6 | |
+ | − | − | + | 19.4 | 0.1 | |
+ | + | − | + | 2.4 | 0.4 | |
+ | − | + | − | 30.6 | 1.1 | |
+ | − | + | + | 69.5 | 5.5 | |
AP-1 | − | − | 1.0 | 0.1 | ||
− | + | 0.2 | 0.0 | |||
+ | − | 1.1 | 0.0 | |||
+ | + | 0.4 | 0.0 | |||
PRL-1 pP1-Sma | − | − | 1.0 | 0.1 | ||
− | + | 1.4 | 0.2 | |||
+ | − | 1.1 | 0.2 | |||
+ | + | 0.7 | 0.1 | |||
HRS/SRp40 pGL0.1 | − | − | 1.0 | 0.2 | ||
− | + | 0.5 | 0.1 | |||
+ | − | 2.0 | 0.4 | |||
+ | + | 0.3 | 0.0 | |||
SMAD7 | − | − | 1.0 | 0.1 | ||
− | + | 1.1 | 0.2 | |||
+ | − | 1.6 | 0.4 | |||
+ | + | 1.5 | 0.4 |
HeLa cells were transfected with the indicated reporter constructs (50 ng) in 24-well plates. For cotransfection experiments, 50 ng of pCMV-STAT3, 50 ng of pCMV-c-fos, 100 ng of DN-STAT3, or 50 ng of pcDNA-HNF-1α (aa 1 to 628, full length) was (+) or was not (−) used. The transfected cells were treated with IL-6 (100 ng/ml) for 4 h. Luciferase activity was expressed as fold induction relative to the basal activity of the reporter construct in the absence of IL-6 treatment and in the absence of any indicated expression plasmids. Nine independent determinants were made for each construct by performing triplicates in three separate experiments.