Table 2.
Comparison of HepG2 cells with hepatocytes and cells with HCC and HB.
| HepG2 Cells | Hepatocyte Cells | Cells with HB | Cells with HCC | |
|---|---|---|---|---|
| Cell size and shape | 12–19 µm, polygonal | 15 µm, cube | 10–20 µm, round or angulated | >10 µm, spindle-shaped and show bizarre anaplastic figures |
| Subcellular components | Large nuclei, 3–7 nucleoli, low mitochondrial content, and poorly developed SER 1 | Two or more nuclei occupy 5–7% of the cell volume; high SER1 and mitochondria | Small, round, inconspicuous nucleoli; low mitochondrial and RER 2 content | The numbers of mitochondria and ER 3 is reduced, and have an abnormal structure, characteristic of stressful conditions |
| Number of chromosomes | 50–60 | Polyploidy | Aneuploidy, >46 |
Aneuploidy, >46 |
| Genome stability, DNA content |
7.5 pg genomic DNA, genome unstable | ~6 pg genomic DNA, stable genome | Genome unstable | Genome unstable |
1 SER, smooth endoplasmic reticulum; 2 RER, rough endoplasmic reticulum; 3 ER, endoplasmic reticulum.