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. Author manuscript; available in PMC: 2021 Dec 9.
Published in final edited form as: Cell Rep. 2021 Oct 5;37(1):109767. doi: 10.1016/j.celrep.2021.109767

Figure 3. Cardiac-specific depletion of PHD2/3 worsens hypertrophic cardiomyopathy.

Figure 3.

Following tamoxifen injection, D2/3_HKO and D2/3_HWT mice were fed with HFD or CC for 12 weeks (n = 6–11 mice group).

(A and B) Echocardiography analyses were performed at 4 weeks (top panel) and 12 weeks (bottom panel) after HFD or CC. HFD fastens the impairment of cardiac function in D2/3_HKO, showing significant decrease of ejection fraction at 4 weeks after tamoxifen infusion, instead of 12 weeks for CC-fed D2/3_HKO mice.

(C and D) Representative pictures of WGA-stained mid-myocardial cross section (top panel), and the resulting quantification (bottom panel; n = 4 per group). Scale bar, 40 μm.

(E and F) Representative pictures of Oil Red O-stained cardiac cross-sections at 12 weeks after HFD or CC and subsequent estimation of lipid deposit area (bottom panel, n = 4 per group). Scale bar, 40 μm.

Data are presented as means ± SEM. Statistical differences after single-factor ANOVA and post hoc analysis were indicated with asterisks: *p < 0.05 versus the control chow or HFD PHD_HWT control group; **0.01 < p < 0.05 versus the corresponding control group; ***p < 0.001 versus the corresponding control group.