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Protocols according to each subgroup.
| Subgroup | Processing Protocol |
|---|---|
| Ap, Ag | Control |
| Bp, Bg | Storing in distilled water for 7 days |
| Cp, Cg | Storing in distilled water + thermal cycling |
| Dp, Dg | Storing in distilled water + thermal cycling + hot coffee staining |
| Ep, Eg | Storing in distilled water + thermal cycling +cold juice bath (5 °C) |
