Table 1.
Precision and accuracy of lyso-Gb3 assay.
| Lyso-Gb3 Amount Spiked in Blank Plasma (ng/mL) |
Intraday (n = 6) | Interday (n = 9) | ||||
|---|---|---|---|---|---|---|
| Calculated Concentration (Mean ± SD) (ng/mL) |
Precision (RSD%) |
Accuracy (%) |
Calculated Concentration (Mean ± SD) (ng/mL) |
Precision (RSD%) |
Accuracy (%) |
|
| 0.25 (LLOQ) | 0.26 (0.02) | 7.7 | 2.6 | 0.25 (0.04) | 5.6 | 2.1 |
| 0.50 | 0.53 (0.02) | 3.8 | 5.2 | 0.52 (0.02) | 3.9 | 3.1 |
| 50.00 | 48.46 (1.30) | 2.7 | −3.1 | 49.44 (2.40) | 4.8 | −1.1 |
| 100.00 | 95.98 (2.48) | 2.6 | −4.0 | 94.85 (5.46) | 5.8 | −5.1 |
Precision (RSD%): 100 × SD/mean; accuracy (%): 100 × (mean concentration found—known concentration)/known concentration); interday (n = 9): triplicate samples for each QC level, over a series of three analyses on different days; LLOQ: lower limit of quantification.