Table 2.
Stability data of lyso-Gb3 assay.
| Lyso-Gb3 Amount Spiked in Blank Plasma (ng/mL) | Stability Test | Mean ± SD (ng/mL) n = 3 |
Precision (%) |
Accuracy (%) |
|---|---|---|---|---|
| 0.50 | 3 h (short stability) | 0.52 ± 0.02 | 3.0 | 4.6 |
| 24 h (autosampler stability) | 0.52 ± 0.03 | 4.7 | 5.6 | |
| 30 days −20 °C (long stability) | 0.53 ± 0.02 | 3.2 | 7.1 | |
| 30 days −80 °C (long stability) | 0.52 ± 0.04 | 7.9 | 3.3 | |
| 100.00 | 2 h (short stability) | 93.68 ± 2.02 | 2.2 | −6.3 |
| 24 h (autosampler stability) | 95.93 ± 2.58 | 2.7 | −4.1 | |
| 30 days −20 °C (long stability) | 106.83 ± 4.05 | 3.8 | 6.8 | |
| 30 days −80 °C (long stability) | 103.29 ± 8.21 | 7.9 | 3.3 |
Precision (RSD%): 100 × SD/mean; accuracy (%): 100 × (mean concentration found—known concentration)/known concentration); LLOQ: lower limit of quantification.