FIG. 10.

The oligomeric complex of onco-Dbl is capable of recruiting multiple Rho GTPases. (A) HA-tagged Cdc42, RhoA, or Rac1 was expressed in Cos-7 cells alone or together with HA-Dbl. The cell lysates were incubated with glutathione-agarose-immobilized GST or GST-N17Cdc42 for 30 min. The input cell lysates and the GST fusion coprecipitates were analyzed in parallel by anti-HA Western blotting. (B) HA-Rac1 was expressed alone, together with wild-type (WT) onco-Dbl, or together with the H556A mutant of onco-Dbl in Cos-7 cells. The cell lysates and the GST or GST-N17Cdc42 coprecipitates from the cell lysates were probed with anti-HA antibody in a Western blot.