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. 2001 Jan;21(2):425–437. doi: 10.1128/MCB.21.2.425-437.2001

FIG. 9.

FIG. 9

Effect of the oligomer-deficient H556A mutant on actin cytoskeletal structure and JNK activation. (A) The morphology and actin structures of H556A mutant-or wild-type (WT) Dbl-expressing NIH 3T3 cells, as well as of the mock-transfected cells, were visualized under a phase-contrast or fluorescence microscope after actin staining with rhodamine-conjugated phalloidin. (B) Various pKH3 constructs (0.4 μg) or controls (pFC-MEKK and pFC2-dbd plasmids) (0.1 μg) were transiently cotransfected into NIH 3T3 cells together with the pFR-Luc reporter plasmid (1 μg) and pFA2-cJun plasmid (0.1 μg). At 48 h posttransfection, the cells were washed and harvested for the measurement of luciferase activities.