Table 4.

Identification of the target enzyme(s) in ergosterol biosynthesis. Heatmap of the detected sterols. The relative sterol amount has been normalized to that of untreated samples [33]. Red: marker sterols according to Müller et al. [3]; boxes in red colour > 10-fold change, in blue colour no changes were observed, and in purple colour <0.1-fold change. A, amorolfine hydrochloride; n.d., not detected; (), trivial name; ?, unknown position of the second hydroxy group. Test concentrations: Aspergillus fumigatus: amorolfine hydrochloride (4.0 µg/mL), 2b (3.5 µg/mL), 3b (4 µg/mL); Candida albicans: amorolfine (4.0 µg/mL), 2b (3.5 µg/mL), 3b (4.0 µg/mL); Candida glabrata: amorolfine hydrochloride (7.5 µg/mL), 2b (3.5 µg/mL), 3b (4.0 µg/mL).

Enzyme	Sterol	Strain	A. fumigatus			C. flbicans			C. glabrata
		Compound	2b	3b	A	2b	3b	A	2b	3b	A
sterol C14- demethylase	14-methylergosta-8,24(28)-dien-3 β ,6 α -diol		n.d.			n.d.			n.d.
	4,4,14-trimethylcholesta-8,24(28)-dien-3β-ol (lanosterol)
	4,4,14-trimethylergosta-8,24(28)-dien-3β-ol (eburicol)
sterol C14- reductase	4,4-dimethylcholesta-8,14,24-trien-3β-ol (FF-MAS)		n.d.			n.d.			n.d.
	ergosta-8,14,24(28)-trien-3β-ol
sterol C4-demethylase complex	4,4-dimethylcholesta-8,24-dien-3β-ol (T-MAS)		n.d.			n.d.			n.d.
	4-methylergosta-8,24(28)-dien-3β-ol
sterol C24-methyltransferase	cholesta-5,7,24-trien-3β-ol		n.d.			n.d.			n.d.
	cholesta-5,7,22,24-tetraen-3β-ol		n.d.			n.d.			n.d.
sterol C8- isomerase	ergosta-5,8,22-trien-3β-ol (lichesterol)
	ergosta-5,8,24(28)-trien-3β-ol
	ergosta-5,8,22,24(28)-tetraen-3β-ol
	ergosta-8,24(28)-dien-3β-ol
	ergosta-8,24(28)-diendi-3β,?-ol (hydroxyfecosterol)
sterol C5- desaturase	ergosta-7,22-dien-3β-ol
	ergosta-7,24(28)-dien-3β-ol (episterol)
	ergosta-7,22,24(28)-trien-3β-ol
sterol C22- desaturase	ergosta-5,7-dien-3β-ol
	ergosta- 5,7,24(28)-trien-3β-ol
sterol C24- reductase	ergosta-5,7,22,24(28)-tetraen-3β-ol		n.d.			n.d.			n.d.
	ergosta-5,7,22-trien-3β-ol (ergosterol)