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. 2021 Nov 28;26(23):7208. doi: 10.3390/molecules26237208

Table 5.

Identification of the target enzyme(s) of compounds 2b and 3b in cholesterol biosynthesis on HL-60 cells. Mainly accumulating sterols under incubation and identified target enzymes. (), trivial name; n.d., not determined [35].

Compound Mainly Accumulating Sterol(s) Inhibited Enzyme(s)
2b 0.1 µM: cholesta-8,14-dien-3β-ol
1 µM: cholesta-8,14-dien-3β-ol, cholesta-8-en-3β-ol (zymostenol),
cholesta-5,7-dien-3β-ol (7-dehy drocholesterol), cholesta-7-en-3β-ol (lathosterol);
10 µM: toxic		 0.1 µM: sterol C14-reductase, sterol C8-isomerase
1 µM: multi-enzyme inhibition
10 µM: n.d.
3b 0.1 µM: no accumulation;
1 µM: cholesta-8,14-dien-3β-ol, cholesta-8-en-3β-ol (zymostenol),
cholesta-5,7-dien-3β-ol (7-dehydrocholesterol), cholesta-7-en-3β-ol (lathosterol);
10 µM: toxic		 0.1 µM: n.d.
1 µM: multi-enzyme inhibition
10 µM: n.d.