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. 2021 Aug 23;1(4):100054. doi: 10.1016/j.xjidi.2021.100054

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

The change of HA localization in TSG-6^–/– RHE seems to be enhanced by TR infection.TSG-6^+/+ and TSG-6^–/– (a and b) RHE infected or not infected with TR and recovered 4 days after infection. (a) PAS staining with α-amylase pretreatment and hemalun counterstaining and HA detection. Bar = 20 μm. (b) HA released in culture medium measured by ELISA-like assay (mean ± SD; n = 4; two-way ANOVA). (c) HA remaining inside RHE (mean ± SD; n = 3; two-way ANOVA). CTL, control; HA, hyaluronan; PAS, periodic-acid schiff; RHE, reconstructed human epidermis; TR, Trichophyton rubrum.