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. 2021 Aug 23;1(4):100054. doi: 10.1016/j.xjidi.2021.100054

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Elevated TSG-6-encoding mRNA expression and protein release in RHE infected by Trichophyton rubrum arthroconidia. RHEs were infected or not infected by T. rubrum arthroconidia and maintained in culture for 4 additional days. (a) PAS staining with α-amylase pretreatment and hemalun counterstaining and (b) HA detection. Bar = 20 μm. (c) TSG-6-encoding mRNA detection using in situ hybridization and hemalun counterstaining. Bar = 20 μm. (d) LL37 mRNA expression assessed by RT-qPCR. The reference gene is RPLP0 (mean ± SD; n = 3; one-way ANOVA; ∗∗P < 0.01 vs. day 0). HA, hyaluronan; PAS, periodic-acid schiff; RHE, reconstructed human epidermis.