Table 1.
Transcriptomic Analysis of Epidermis Reconstructed from N/TERT Cells and Stimulated or Not with IL-4 and IL-13 through RNA Sequencing
| Gene | Name | log2FC | Padj |
|---|---|---|---|
| CISH | Cytokine inducible SH2 containing protein | 3.07684025 | 0.000E+00 |
| ANO1 | Anoctamin 1 | 2.80591740 | 1.042E–219 |
| SLC16A14 | Solute carrier family 16 member 14 | 4.47550706 | 8.627E–192 |
| CA2 | Carbonic anhydrase 2 | 2.18654582 | 1.411E–188 |
| CTSC | Cathepsin C | 1.54462245 | 5.680E–123 |
| GLDC | Glycine decarboxylase | 6.48425833 | 7.039E–122 |
| SLC26A2 | Solute carrier family 26 member 2 | 1.42119675 | 7.896E–113 |
| AC100801.2 | 2.67582484 | 4.386E–86 | |
| C1QTNF1 | C1q and TNF related 1 | 2.76558625 | 2.474E–85 |
| TNFAIP6 | TNF alpha-induced protein 6 | 4.61410057 | 1.402E–80 |
| BCL2L15 | BCL2 like 15 | 4.38693821 | 1.861E–75 |
| DIO2 | Iodothyronine deiodinase 2 | –1.38620973 | 8.042E–75 |
| ST6GAL1 | ST6 beta-galactoside alpha-2,6-sialyltransferase 1 | 1.94904986 | 5.983E–73 |
| EGR3 | Early growth response 3 | –1.43824080 | 1.248E–70 |
| KRT16 | Keratin 16 | 1.76615476 | 1.724E–67 |
| HRH1 | Histamine receptor H1 | 2.04522544 | 1.800E–60 |
| POSTN | Periostin | 2.96569063 | 9.221E–60 |
| ETS2 | ETS proto-oncogene 2, transcription factor | –0.97990996 | 3.121E–59 |
| EGLN3 | Egl-9 family hypoxia inducible factor 3 | 1.90222718 | 7.334E–58 |
| APOL6 | Apolipoprotein L6 | 1.11187734 | 1.488E–57 |
| LBH | LBH regulator Of WNT signaling pathway | 1.63185691 | 3.760E–57 |
| MXRA5 | Matrix remodeling associated 5 | –1.75646450 | 7.512E–56 |
| RGMB | Repulsive guidance molecule BMP coreceptor B | 1.27097907 | 8.026E–56 |
| NEK6 | NIMA related kinase 6 | 1.46907088 | 4.158E–55 |
| C1orf68 | Chromosome 1 open reading frame 68 | –2.17535843 | 5.709E–54 |
| NELL2 | Neural EGFL like 2 | 2.77669873 | 1.809E–52 |
| PTGS2 | Prostaglandin-endoperoxide synthase 2 | –2.48531923 | 2.546E–51 |
| GCNT3 | Glucosaminyl (N-acetyl) transferase 3, mucin type | 2.13974316 | 1.036E–50 |
| MAP3K14 | Mitogen-activated protein kinase 14 | 1.62240356 | 1.088E–50 |
| DUOX2 | Dual oxidase 2 | 2.12196856 | 3.027E–50 |
| PADI3 | Peptidyl arginine deiminase 3 | 1.83977015 | 5.216E–50 |
| CD59 | CD59 molecule (CD59 blood group) | 0.75327573 | 1.087E–49 |
| KRT4 | Keratin 4 | 1.99977131 | 1.734E–49 |
| BMP4 | Bone morphogenetic protein 4 | 2.53670445 | 1.151E–47 |
| BTBD3 | BTB domain containing 3 | 0.78079374 | 1.162E–47 |
| AREG | Amphiregulin | –1.47407342 | 7.747E–47 |
| PDE6A | Phosphodiesterase 6A | 2.22277344 | 1.398E–46 |
| DENND4A | DENN domain containing 4A | 0.83293501 | 1.443E–44 |
| CCN1 | Cellular communication network factor 1 | –1.81412009 | 2.467E–44 |
| SORL1 | Sortilin related receptor 1 | 0.96862192 | 5.845E–44 |
| BCL10 | BCL10 immune signaling adaptor | –1.01814850 | 9.519E–44 |
| RASGRP1 | RAS guanyl releasing protein 1 | 1.15867682 | 1.093E–43 |
| CXCL2 | C-X-C motif chemokine ligand 2 (IL-2) | –3.20712371 | 7.322E–43 |
| HBEGF | Heparin-binding EGF like growth factor | –1.92196976 | 1.046E–42 |
| FLT1 | Fms related receptor tyrosine kinase 1 | 2.52370748 | 1.357E–42 |
| LGALSL | Galectin like | –0.93501839 | 3.556E–42 |
| CASP14 | Caspase 14 | –1.20133651 | 6.805E–41 |
| CTSH | Cathepsin H | –1.48572279 | 8.798E–40 |
| TXN | Thioredoxin | 1.09419641 | 2.633E–39 |
Abbreviations: Padj, adjusted P; FC, fold change; RHE, reconstructed human epidermis.
List of 50 more regulated genes in IL-4‒ and IL-13‒challenged RHE compared with nonstimulated RHE based on Padj-value.