Table 1.
Method validation data for vanadium (V) in plasma
| Parameter | Result |
|---|---|
| Concentration range (ng V/mL plasma) | 5.00 – 1,000 |
| Correlation coefficient (r) | > 0.99 |
| Matrix standard accuracy (%RE) | −1.4 – 5.1% |
| LODa (ng V/mL plasma) | 0.268 |
| LOQb (ng V/mL plasma) | 5.00 |
| Selectivity (mean matrix blank response relative to LOQ, %) | 17.3% |
| Mean Instrument drift (%) | 16.4% |
| Absolute recovery (%) | 110 – 122% |
| Precision and accuracyc | |
| Intra-day accuracy (mean %RE) | 8.5 – 15.6% |
| Intra-day precision (%RSD)d | ≤ 1.8% |
| Inter-day accuracy (mean %RE) | 7.3 – 11.7% |
| Inter-day precision (%RSD) | ≤ 3.1% |
| Dilution verification (up to 7,500 ng V/mL plasma) | |
| Accuracy (mean %RE) | 5.6% |
| Precision (%RSD) | 0.9% |
a
LOD = Limit of detection.
b
LOQ = Lower limit of quantitation, or the lowest calibration standard that exhibited −20% to 20% relative error (RE).
c
Precision and accuracy were determined for triplicate QC samples fortified at three levels, 7.50, 250, and 800 ng V/mL plasma.
d
%RSD = percent relative standard deviation.