TABLE 1.
Oligodeoxyribonucleotide primers used to amplify the 16S rRNA gene sequence of H. mesocricetorum
| Primer | Sequence (5′ to 3′)a | Positionb |
|---|---|---|
| Broad range bacterial | ||
| C70f | AGA GTT TGA TYM TGG C | 8–23 |
| B37r | TAC GGY TAC CTT GTT ACG A | 1513–1495 |
| Helicobacter sequencing | ||
| H419r | AAT CCT AAA ACC TTC ATC CTC | 419–406 |
| H593f | TAA GTC AGA TGT GAA ATC C | 593–611 |
| Helicobacter genus specific | ||
| H276f | TAT GAC GGG TAT CCG GC | 277–293 |
| H676r | ATT CCA CCT ACC TCT CCC A | 676–658 |
| H. mesocricetorum specific | ||
| Hm197f | GAG GGA AAG TTT TTC GCT ATG A | 197–225 |
| Hm859r | TGC ATT ACT GCA AAG ACA AGC TT | 859–844 |
a
Standard International Union of Biochemistry codes for bases and ambiguity.
b
The positions within the Escherichia coli 16S rRNA gene sequence that correspond to the 5′ and 3′ ends of each primer are shown. Approximate positions are given for Helicobacter-specific primers.