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. 2021 May 6;1(2):100020. doi: 10.1016/j.xjidi.2021.100020

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Effect of dHACM on TGFβ1-mediated αSMA synthesis. (a) mRNA level of αSMA in HDFs normalized to those of 18S and expressed as a fold change compared with that of TGFβ1. (b) Expression of αSMA in fibroblasts assessed by western blot analysis. All values represent mean ± SD. ∗P < 0.05 versus TGFβ1 group and #P < 0.05 versus basal group using one-way ANOVA; n = 3 dHACM donors. (c) Representative IF staining for αSMA. Bar = 100 μm. dHACM, dehydrated human amnion/chorion membrane; HDF, human dermal fibroblast; IF, immunofluorescence; αSMA, alpha-smooth muscle actin.