Skip to main content
. 2021 May 6;1(2):100020. doi: 10.1016/j.xjidi.2021.100020

Figure 4.

Figure 4

dHACM suppresses TGFβ1-stimulated contraction of fibroblasts. (a) Representative images of collagen gels. (b) After 48 hours, the diameter of each collagen gel was measured. Treatments include no cells (negative control), media alone (vehicle control), 20 ng/ml TGFβ1 (positive control), 20 mg/ml dHACM + 20 ng/ml TGFβ1, 10 mg/ml dHACM + 20 ng/ml TGFβ1, and 1 mg/ml dHACM + 20 ng/ml TGFβ1. All values represent mean ± SD. ∗P < 0.05 versus vehicle control and #P < 0.05 versus positive control using one-way ANOVA; n = 3 dHACM donors. (c) Representative IF staining for αSMA. Bar = 100 μm. dHACM, dehydrated human amnion/chorion membrane; IF, immunofluorescence.