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Control IF of collagen gels. Collagen gels treated with media alone (vehicle control), 20 ng/ml TGFβ1 (positive control), 20 mg/ml dHACM + 20 ng/ml TGFβ1, 10 mg/ml dHACM + 20 ng/ml TGFβ1, and 1 mg/ml dHACM + 20 ng/ml TGF-β1. Representative images of IF control staining using (a) mouse IgGa2a and (b) secondary antibody only. Bar = 100 μm. dHACM, dehydrated human amnion/chorion membrane; IF, immunofluorescence.
