Figure 5.

Features of circulating ILC2s and skin-resident ILC2s. (a) Flow cytometry of ILC2s from the lymph node (thin line) and skin (bold line) of IL33tg mice. Cells were gated on lineage marker (Lin)⁻ Sca-1⁺ IL-33R (ST2)⁺ cells for ILC2s. (b) Flow cytometry of cells from the skin of IL33tg mice. The cells were gated on an ILC2 fraction. Note that the ICOS⁺ KLRG1⁺ IA/IE⁺ cells are circulating ILC2s, and the ICOS⁺ KLRG1^‒ IA/IE^‒ cells are skin-resident ILC2s. (c) Immunofluorescence of IA/IE and ICOS in Rag2 knockout IL33tg mouse skin. Note that the ICOS⁺ IA/IE⁺ cells (red/green double‒positive cells, arrowheads) are circulating ILC2s, and the ICOS⁺ IA/IE^‒ cells (green single‒positive cells) are skin-resident ILC2s. Representative data are from three independent experiments. Bar = 100 μm (inset, 10 μm). ILC2, group 2 innate lymphoid cell; Max, maximum.