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. 2021 Jul 2;1(3):100035. doi: 10.1016/j.xjidi.2021.100035

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© 2021 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Gating strategy for flow cytometry. (a) Gating strategy for the analysis of FSC^lowSSC^lowCD45⁺ lineage (Lin)⁻Thy1.2⁺Sca-1⁺ IL-33R (ST2)⁺ ILC2s. The numbers indicate the percentage of cells in each gate. (b) Gating strategy for the analysis of CD45⁺ B220⁻ DCs within the draining LNs 72 hours after the photoconversion of the skin. The LN-resident DCs are CD11c^high IA/IE^med, and the skin-derived DCs are CD11c^med IA/IE^high. Note that only skin-derived DCs contain KikGR-Red⁺ cells. The numbers indicate the percentage of cells in each quadrant. Similar results were obtained from two independent experiments. DC, dendritic cell; ILC2, group 2 innate lymphoid cell; KikGR, Kikume Green-Red; LN, lymph node.