FIG. 2.

Wild-type and mutant RAG-1 proteins bind to good and bad flanks similarly. Radiolabeled oligonucleotide substrates were incubated in Ca²⁺ with RAG-1 proteins as indicated. The positions of the unbound substrate and the protein-bound complex are indicated. (A) Binding to a good flank 12-RSS substrate (lanes 1 to 4) and a bad coding flank 12-RSS substrate (lanes 5 to 8). (B) Binding to a bad coding flank substrate (lanes 1 to 4) and a nonspecific oligonucleotide control (lanes 5 to 8).