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. 2021 Nov 15;10:e73753. doi: 10.7554/eLife.73753

Figure 1. Water extract of Gastrodia elata Blume (WGE) treatment rescues the diminished locomotion of Ddc>G2019S flies.

(A–C) Climbing activities of Ddc>G2019S flies fed on food supplemented with 0.1, 0.5, or 1.0% WGE (A), 0.1 or 1.0 mM gastrodin (B), and 0.1 or 1.0 mM 4-hydroxybenzyl alcohol (4-HBA) (C). Controls are Ddc>Lrrk2 and Ddc>G2019S flies fed regular food. Bar graphs show the percentages of flies (mean ± SEM, N = 6) that climbed above 8 cm within 10 s. (D) Five-minute walking tracks pooled from eight flies each of the Ddc>Lrrk2, Ddc>G2019S, and Ddc>G2019S + 0.1% WGE groups at week 4. Bar graph at right summarizes their walking distances (mean ± SEM, N = 8). One-way analysis of variance (ANOVA) with Tukey’s post-hoc multiple comparison test: *p<0.05, **p<0.01, and ***p<0.001, ns, not significant.

Figure 1.

Figure 1—figure supplement 1. Climbing activity assay of Ddc>mCD8-GFP and Ddc>Lrrk2 flies.

Figure 1—figure supplement 1.

(A) Comparable climbing activities were detected for Ddc>mCD8-GFP and Ddc>Lrrk2 flies. Both exhibited better climbing activities than Ddc>G2019S flies in the climbing assay from weeks 1 to 6. Bar graph shows percentage (mean ± SEM, N = 5) of flies that successfully climbed above 8 cm within 10 s. One-way analysis of variance (ANOVA) and Tukey’s post-hoc multiple comparison test: *p<0.05, ***p<0.001, ns, not significant. (B) Ddc>G2019S flies fed with 0.02 or 0.1% water extract of Gastrodia elata Blume (WGE) exhibited improved climbing activity (mean ± SEM, N = 6). One-way ANOVA and Tukey’s post-hoc multiple comparison test: *p<0.05, ***p<0.001 (relative to Ddc>G2019S); and ##p<0.01, ###p<0.001 (comparing different doses of WGE), ns, not significant.
Figure 1—figure supplement 2. Locomotion improvement of Ddc>G2019S flies starting water extract of Gastrodia elata Blume (WGE) feeding at week 4.

Figure 1—figure supplement 2.

The climbing activities of Ddc>Lrrk2, Ddc>G2019S, and Ddc>G2019S with 0.1% WGE feeding at week 4 were assessed at weeks 3–6. Bar graphs show success rates (mean ± SEM, N = 6) of flies climbing over 8 cm height in 10 s. One-way ANOVA and Tukey’s post-hoc multiple comparison test were performed and statistical significance is shown as ***p<0.001, *p<0.05, ns, not significant.