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. 2021 Nov 15;10:e73753. doi: 10.7554/eLife.73753

Figure 3. Water extract of Gastrodia elata Blume (WGE) modulates Lrrk2 accumulation and hyperactivation in elav>G2019S flies.

(A) Representative immunoblots of 3-day-old adult brain lysates showing expression levels of Lrrk2, pLrrk2 (phosphorylated at Ser1292), Akt, and pAkt (phosphorylated at Ser505) in elav>Lrrk2 and elav>G2019S flies. (B, C) Quantifications (mean ± SEM, N = 3) of Lrrk2 and pLrrk2/Lrrk2 (B), and Akt and pAkt/Akt (C). (D) Representative immunoblots of 2- and 4-week-old adult brain lysates showing Lrrk2 levels in the fly groups elav>Lrrk2, elav>G2019S, and elav>G2019S fed with 0.1% WGE. (E) Quantification of Lrrk2 levels in 2- and 4-week-old adult brains (mean ± SEM, N = 3). (F) Representative immunoblots of 4-week-old adult brain lysates showing expression levels of Lrrk2, pLrrk2, Rab10, and pRab10 (phosphorylated at Thr73). (G, H) Quantification (mean ± SEM, N = 3) of levels of Lrrk2 and pLrrk2/Lrrk2 (G), and of Rab10 and pRab10/Rab10 (H). One-way ANOVA with Tukey’s post-hoc multiple comparison test: *p<0.05, **p<0.01, ns, not significant.

Figure 3.

Figure 3—figure supplement 1. Water extract of Gastrodia elata Blume (WGE) specifically modulates Lrrk2 accumulation and hyperactivation in elav>G2019S but not elav> Lrrk2 flies.

Figure 3—figure supplement 1.

(A) Representative immunoblots of 2-week-old adult brain lysates showing levels of Lrrk2 and pLrrk2 (Ser1292) in elav>Lrrk2, WGE-fed elav>Lrrk2, elav>G2019S, and WGE-fed elav>G2019S flies. (B, C) Quantification (mean ± SEM, N = 3) of Lrrk2 (B) and pLrrk2/Lrrk2 (C) levels. One-way ANOVA and Tukey’s post-hoc multiple comparison test: *p<0.05, ***p<0.001, ns, not significant.