(A) Representative immunoblots of 2- and 4-week-old adult brain lysates showing levels of Akt and pAkt in brain extracts of elav>Lrrk2, elav>G2019S, and WGE-fed elav>G2019S flies. (B) Quantification (mean ± SEM, N = 3) of pAkt/Akt levels in 2- and 4-week-old adult brains. (C) Representative immunoblots of 4-week-old adult brain lysates showing levels of Nrf2, pNrf2 (phosphorylated at Ser40), GSK3β, pGSK3β (phosphorylated at Ser9), and HO-1 in elav>Lrrk2, elav>G2019S, and WGE-fed elav>G2019S flies. GADPH acted as a loading control in (A) and (C). (D) Quantification (mean ± SEM, N = 3) of relative protein levels to respective Nrf2, GSK3β, and HO-1. One-way ANOVA with Tukey’s post-hoc multiple comparison test (relative to elav>G2019S): *p<0.05, **p<0.01, ***p<0.001, ns, not significant.