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. 2021 Nov 15;10:e73753. doi: 10.7554/eLife.73753

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© 2021, Lin et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 7. — (A) Representative images of glial pMad staining in the adult PPL1 clusters of Ddc>Lrrk2, Ddc>G2019S, and WGE-fed Ddc>G2019S flies. White arrows indicate pMad signals co-localized with Repo, with single channels for pMad signals shown as insets. Bar: 10 μm. (B) Quantification (mean ± SEM, n > 60 for each genotype) of pMad signals normalized to Repo levels in glia of the indicated genotypes. One-way ANOVA and Tukey’s post-hoc multiple comparison test (relative to Ddc>G2019S): ***p<0.001. (C, D) Climbing success rates (mean ± SEM, N = 10) at weeks 1–4 demonstrating that WGE treatment rescues locomotion deficits induced by glial overexpression of Mad (C) or tkv^Q253D (D) in Tub-GAL80^ts; repo-GAL4 flies. One-way ANOVA and Tukey’s post-hoc multiple comparison test (relative to repo>Mad or repo>tkvQ^Q253D): **p<0.01, ***p<0.001. (E, G) Representative images of 4-week-old adult brain showing TH staining of the PPL1 clusters of Tub-GAL80^ts; repo-GAL4, Tub-GAL80^ts; repo>Mad, and WGE-fed Tub-GAL80^ts; repo>Mad flies (E), and Tub-GAL80^ts; repo-GAL4, Tub-GAL80^ts; repo>tkvQ^Q253D, and WGE-fed Tub-GAL80^ts; repo>tkvQ^Q253D flies (G). Bars: 12.5 μm. (F, H) Bar graphs show mean ± SEM (N = 5) of TH-positive dopaminergic neurons in the PPL1 clusters of 4-week-old flies. One-way ANOVA and Tukey’s post-hoc multiple comparison test (relative to Tub-GAL80^ts; repo>Mad [F] or Tub-GAL80^ts; repo>tkvQ^Q253D [H]): ***p<0.001.

Figure 7—figure supplement 1. — (A) Representative images of glial pMad staining in the adult PPL1 clusters of Ddc>Lrrk2, Ddc>G2019S, and WGE-fed Ddc>G2019S flies. White arrows indicate pMad signals co-localized with Repo, with single channels for pMad signals shown as insets. Bar: 10 μm. (B) Quantification (mean ± SEM, n > 60 for each genotype) of pMad signals normalized to Repo levels in glia of the indicated genotypes. One-way ANOVA and Tukey’s post-hoc multiple comparison test (relative to Ddc>G2019S): ***p<0.001. (C, D) Climbing success rates (mean ± SEM, N = 10) at weeks 1–4 demonstrating that WGE treatment rescues locomotion deficits induced by glial overexpression of Mad (C) or tkv^Q253D (D) in Tub-GAL80^ts; repo-GAL4 flies. One-way ANOVA and Tukey’s post-hoc multiple comparison test (relative to repo>Mad or repo>tkvQ^Q253D): **p<0.01, ***p<0.001. (E, G) Representative images of 4-week-old adult brain showing TH staining of the PPL1 clusters of Tub-GAL80^ts; repo-GAL4, Tub-GAL80^ts; repo>Mad, and WGE-fed Tub-GAL80^ts; repo>Mad flies (E), and Tub-GAL80^ts; repo-GAL4, Tub-GAL80^ts; repo>tkvQ^Q253D, and WGE-fed Tub-GAL80^ts; repo>tkvQ^Q253D flies (G). Bars: 12.5 μm. (F, H) Bar graphs show mean ± SEM (N = 5) of TH-positive dopaminergic neurons in the PPL1 clusters of 4-week-old flies. One-way ANOVA and Tukey’s post-hoc multiple comparison test (relative to Tub-GAL80^ts; repo>Mad [F] or Tub-GAL80^ts; repo>tkvQ^Q253D [H]): ***p<0.001.