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. 2021 Dec 8;41(49):10148–10160. doi: 10.1523/JNEUROSCI.1152-21.2021

Figure 4.

Figure 4.

Learning of the recessed forelimb reach task was unimpaired following DT-induced ablation of DTR-expressing cholinergic neurons. In a subset of experiments, hemizygous ChAT-Cre mice were co-injected with AAV8-syn-FLEX-DTR and AAV2-syn-FLEX-EGFP. Two weeks later, mice were injected with DT. Confocal images showed EGFP expression in mice with or without transduction of DTR virus (A). Scale bar: 200 μm. B, Quantification of the number of EGFP-positive neurons in basal forebrain of animals injected with AAV8-DTR as compared with control animals (unpaired t test, ***p < 0.001). C, Genetic ablation of cholinergic neurons did not impair learning of recessed forelimb reach task (repeated-measures ANOVA, p = 0.1429, F(1,11) = 2.49). Data presented as mean ± SEM.