Figure 8.

Basal forebrain cholinergic neurons are not required for execution of previously learned coordinated motor behavior. A, Timeline outlining experimental details of cholinergic ablation by p75-SAP following rotarod training. Retention testing was performed two weeks after saporin injection. B, Pharmacological ablation of cholinergic neurons did not affect the performance on the previously learned rotarod behavior (paired t tests, p > 0.05 for both groups). C, The ratio of latencies to fall before and after cholinergic ablation (post/pre; two-tailed t test, p = 0.34). D, Timeline outlining experimental details of cholinergic ablation by AAV-DTR following rotarod training. Rotarod retention testing was performed two weeks after DT injection. E, Genetic ablation of cholinergic neurons did not affect the performance on the previously learned rotarod behavior (paired t tests, p > 0.05 for both groups). F, The ratio of latencies to fall before and after DT-induced cholinergic ablation (post/pre; two-tailed t test, p = 0.24). Data presented as mean ± SEM.