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. 2021 Nov 10;8:762363. doi: 10.3389/fnut.2021.762363

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Copyright © 2021 Lin, Chen, Chen, Lin, Wu, Juan and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GlcN supplementation expands the compartment of MDSCs. (A) Schematic of PMN-MDSC and M-MDSC flow cytometry gating. PMN-MDSC and M-MDSC levels in (B) peripheral blood (PB), (C) bone marrow (BM), (D) spleen (SP) following i.p. injection. (E) PMN-MDSC and M-MDSC levels in the PB following pump release. Wild-type BM cells were stimulated with IL-6, GM-CSF, G-CSF, and transforming growth factor-β (TGF-β) to induce MDSC expansion in vitro. (F) Expanded CD11b⁺Gr1⁺ MDSC frequency. (G) Expanded PMN-MDSC frequency. (H) Expanded M-MDSC frequency. The quantified results are presented as the mean ± standard deviation. *P < 0.05; **P < 0.01; ***P < 0.001.