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. 2021 Nov 10;8:762363. doi: 10.3389/fnut.2021.762363

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Copyright © 2021 Lin, Chen, Chen, Lin, Wu, Juan and Chang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GlcN treatment enhances the immunosuppressive ability of MDSCs. Mice were treated with GlcN by i.p. injection for 14 days. Arg-1, iNOS, and COX2 expression levels in (A) total BM cells and (B) BM CD11b⁺Gr1⁺ MDSCs. ROS levels in (C) PMN-MDSCs and (D) M-MDSCs isolated from BM, SP, and PB. (E) T cell immunosuppressive ability of BM CD11b⁺Gr1⁺ MDSCs. (F) Quantification of PD-L1 expression levels in BM cells. MFI: Mean fluorescence intensity. Quantified results are presented as the mean ± standard deviation. *P < 0.05; **P < 0.01; ***P < 0.001.