Figure 1.

Schematic representation of the in vitro evolution experiment

A total of 48 populations, quadruplicates of each of the 12 strains, were grown with increasing concentrations of flz (FLZ samples), ani (ANI), both drugs in combination (ANIFLZ), and no drug (YPD). Subsequently, ANI samples were grown in flz (AinF), whereas FLZ samples were grown in ani (FinA). The experiment involved batch serial transfer of the samples every 3 days, in which every second passage involved an increase in drug concentrations up to 4 and 196 μg/mL ani and flz, respectively (Table S4; STAR Methods). After the final passage, an aliquot was plated for single colony isolation and storage.