Fig. 2.

Immunogenicity of RVG-saRNA loaded SLNs, PNPs and LNPs. Humoral immune responses elicited by RVG-saRNA formulated in either DOTAP-based SLNs, PNPs or LNPs following intramuscular (IM, 0.15 μg), intradermal (ID, 0.15 μg) or intranasal (IN, 1.5 μg) administration in mice. Mice were also immunized with benchmark iLNPs [11] or 2% (IM and ID) or 5% (IN) of the human dose of Rabipur. Mice were vaccinated four weeks apart and total anti-RVG IgG titers were quantified four weeks after the first vaccination (A), two weeks after the second vaccination (B) and 10 weeks after the second vaccination (C). Markers depict measurements from pools of 2 mice each. The solid lines represent the geometric mean titer of each group (n = 4–5). Dotted lines at 0.5 and 0.125 EU/mL correspond to the correlate of protection and limit of quantification, respectively.