Fig. 3.

Cellular immune response elicited by RVG-saRNA loaded nanoparticles after IM, ID or IN administration. Splenic CD8+ and CD4+ T cell responses elicited by RVG-saRNA formulated in either DOTAP-based SLNs, PNPs or LNPs following intramuscular (IM, 0.15 μg), intradermal (ID, 0.15 μg) or intranasal (IN, 1.5 μg) administration in mice. Mice were also immunized with benchmark iLNPs [11] and either 2% (IM and ID) or 5% (IN) of the human dose of Rabipur. Splenocytes were collected two weeks after the second vaccination and re-stimulated in vitro with an RVG peptide pool. A) Frequencies of cytokine-producing CD8+ T cells. B) Frequencies of CD107+ CD8+ T cells. C) Frequencies of CD4+ T cells expressed as Th1 and Th0 according to the cytokines expressed. Results are represented as mean ± SD of three samples. Refer to Fig. S1 in the supplemental material for the gating strategy.