Fig. 4.

Lung CD8+ and CD4+ T cell responses following intranasal vaccination. Lung cells were collected two weeks after the second vaccination and re-stimulated in vitro with an RVG peptide pool. A) Frequencies of cytokine-producing CD8+ T cells. B) Frequencies of CD107+ CD8+ T cells. C) Frequencies of CD4+ T cells expressed as Th1 and Th0 according to the cytokines expressed. Results are represented as mean ± SD of three samples. Refer to Fig. S2 in the supplemental material for the gating strategy.