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. 2000 May;38(5):1832–1838. doi: 10.1128/jcm.38.5.1832-1838.2000

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FIG. 4 — In-gel and Southern hybridization. (A) EcoRI-digested DNAs of the donors, recipients, and transconjugants were probed with an ermA gene-specific internal oligonucleotide probe. Lanes 1 and 11, digoxigenin-labeled DNA molecular size marker III (Boehringer Mannheim); lane 2, p58; lane 3, c657; lane 4, p58-c657; lane 5, c803; lane 6, p58-c803; lane 7, c18; lane 8, p58-c18; lane 9, c716; lane 10, p58-c716. (B) Southern hybridization of the donor and transconjugant total DNA with an ermC gene-specific internal oligonucleotide probe. Lanes 1 and 25, control plasmid pE194 DNA; lane 2, p45; lane 3, p46; lane 4, p58; lane 5, p62; lane 6, p63; lane 7, p45-c16; lane 8, p45-c772; lane 9, p58-c657; lane 10, p62-c656; lane 11, p62-c660; lane 12, p62-c661; lane 13, p62-c720; lane 14, p62-c722; lane 15, p63-c18; lane 16, p46-c716; lane 17, p58-c803; lane 18, p62-c712; lane 19, p45-c29; lane 20, p45-c722; lane 21, p46-c796; lane 22, p58-c714; lane 23, p63-c29; lane 24, p58-c716. Designations are explained in the legend to Fig. 3.