FIG. 6.

Schematic representation of NCS1 null alleles and phenotypic analysis of ncs1Δ::LEU2 noh1Δ::HIS3 double-mutant strains. (A) Schematic representation of the NCS1 alleles, ncs1Δ::LEU2 and ncs1Δ::HIS3, used throughout this study. The putative translation start site is shown with an arrow. The termination codon is denoted by an asterisk. (B) Analysis of ncs1Δ noh1Δ double mutants. Tetrads dissected from the meiotic progeny of diploid MATa/MATα ncs1Δ::LEU2/NCS1 NOH1/noh1Δ::HIS3 were placed on YEPD medium and incubated at 30°C for 3 days. (C) Phenotypic analysis of cells lacking PTPA function. The budding index (percent budded cells/total number of cells) of asynchronously growing cultures was measured for strains SY3357, SY3383, and SY3382 after growth of cultures in YEPD medium at 25°C followed by a shift to 37°C for 5 h. WT, wild type.