Ncs1p coimmunoprecipitates with Sit4p from whole-cell extracts (WCE). Lane 1, SY3357 (wild type; WCE); lane 2, SY3357 (wild type; immunoprecipitate [IP]); lane 3, SY3394 (ncs1Δ) carrying YEp13NCS1-3xMYC (IP); lane 4, SY3394 (ncs1Δ) carrying YEp13NCS1-3xMYC (WCE); lane 5, SY3394 (ncs1Δ) carrying YEp13NCS1-3xMYC plus YEp24PPH21-HA (IP); lane 6, SY3394 (ncs1Δ) carrying YEp13NCS1-3xMYC plus YEpURA3ADH-SIT4-HA (IP).