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. 2021 Nov 29;13(22):24542–24559. doi: 10.18632/aging.203724

Figure 3.

Figure 3

Chondrogenic differentiation ability of PDMSCs from different maternal age group. (A) Representative image of chondrogenic differentiation of all 5 age groups. Upper panel showed that undifferentiated cells did not retain Alcian blue staining. Lower panel showed that differentiated cells were positive to Alcian blue staining. Real Time PCR performed to measure chondrocyte markers gene expression of (B) Aggrecan (ACAN) and (C) SOX9 of all 5 age groups. Group A: 18-21; Group B: 22-25; Group C: 26-30; Group D: 31-35; Group E: 36 and over. *, p<0.05; **, p<0.01; ***, p<0.0005; ****, p<0.0001. Scale bar 200 μm.